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HPLC determination of nitrazepam and its metabolites in human urine
Summary
Type:
HPLC
Application #:
104530
Catalog#:
720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection:
UV, 254nm
Conditions:
eluent: methanol - water (35:65) adjusted to pH 4.0 with phosphoric acid
flow rate 1.3ml/min
column temperature 50°C
pressure 120kg/cm2
Authors:
Kozu T
Source:
J Chromatogr 310 (1984) 213 - 218
Details
Legend: Fig. 2. Typical chromatogram of nitrazepam and metabolites, 1. 7-aminonitrazepam, 2. 7-acetamidonitrazepam, 3. nitrazepam, 4. nimetazepam (int. std.). Fig. 3. Chromatogram of the extract of human u
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